DurA Cycle A50

DurA Cycle A50 is a next-gen affinity resin. It is specifically engineered to purify monoclonal antibodies and associated protein constructs while meeting the rigorous demands of modern cleaning-in-place (CIP) protocols. By utilizing a high-capacity 50 µm bead manufactured through patented Jetting technology, this resin ensures a uniform particle size that enhances overall quality and performance. The design addresses common downstream purity challenges by offering mild pH elution up to pH 4.5. This significantly reduces the risk of protein aggregation compared to standard affinity resins. DurA Cycle A50 is built for longevity. Its resistance to protein A leakage and high alkaline stability (up to 0.5 M NaOH) extends the resin’s lifetime, effectively lowering the cost per gram of the product.
Beyond its technical performance, this resin supports more efficient and sustainable manufacturing operations through several key advantages:
  • High Productivity: Delivers a high dynamic binding capacity of up to 80 g/L.
  • Operational Efficiency: The Jetting manufacturing process involves fewer production steps than traditional methods, helping to accelerate your time to market.
  • Sustainability: Jetting technology reduces environmental impact by minimizing the use of water and harmful solvents during production.
  • Enhanced Stability: Engineered to maintain product integrity even under variable and enhanced cleaning strategies.

PraestoTM 70 CH1

Praesto 70 CH1 is a specialized chromatography resin engineered to address the complexities of modern therapeutic pipelines, specifically targeting CH1 antibody domains to simplify the purification of bispecifics and antibody fragments. As the variety of therapeutic molecules expands, traditional purification methods often prove inefficient; however, this resin offers up to twice the dynamic binding capacity of established CH1 alternatives. By providing a high dynamic binding capacity of up to 50 g/L on a 70 µm jetted bead, it significantly improves process economics and helps overcome impurity challenges in complex procedures.
Furthermore, Praesto 70 CH1 is designed for seamless integration into existing workflows while promoting sustainable manufacturing:
  • Standardized Cleaning: Improved alkaline stability (up to 0.1 M NaOH) allows for the adoption of standard CIP protocols, ensuring better resin maintenance.
  • Superior Quality: Manufactured using patented Jetting technology, the resin features a uniform particle size that ensures consistent and enhanced performance.
  • Accelerated Timelines: The Jetting process involves fewer production steps than traditional methods, helping to reduce time to market for critical therapies.
  • Eco-Friendly Production: The manufacturing technique supports sustainability goals by reducing water usage and the reliance on harmful solvents.

Praesto™ AP+80 & Praesto™ AP+50

The Praesto AP+ platform provides a cost-effective solution for drug developers facing the dual challenge of controlling production costs while maintaining high efficacy standards. This resin series is specifically designed for the purification of a wide range of monoclonal antibodies (mAbs) and related constructs, allowing for easy implementation into existing facilities and operational parameters. Whether improving novel or legacy processes, the platform enhances manufacturing efficiencies and accelerates the timeline to clinic. By utilizing patented Jetted Technology, these resins offer uniform particle sizes that ensure enhanced performance and consistent quality.

The platform offers two distinct options to suit different processing needs:

  • Praesto AP+80: An 80 µm protein A resin that combines good capacity with excellent pressure-flow properties, designed for speed and easy implementation at conventional column bed heights.
  • Praesto AP+50: A high-capacity 50 µm protein A resin that delivers maximum resin utilization to support process intensification and superior impurity clearance.

Advantages:

  • Alkaline Stability: Both resins are alkaline stable (0.1 M–0.5 M NaOH), ensuring robust performance during rigorous cleaning-in-place protocols.
  • Sustainability and Speed: The Jetting manufacturing process supports environmental goals by reducing the use of water and harmful solvents, while also accelerating time to market through fewer production steps.

Purolite - Praesto® Jetted A50

Praesto Jetted A50 is a time-tested affinity resin. It is designed for the purification of monoclonal antibodies (mAbs) and associated protein constructs. Over the last four decades, affinity chromatography has become the foundation of therapeutic medicine development. Praesto Jetted A50 resin continues that legacy by supporting various FDA-approved antibody-based therapies. Recognized as the first jetted agarose resin, it prioritizes both sustainability and productivity to meet the evolving needs of the biopharmaceutical industry. By utilizing a high-capacity 50 µm bead, Praesto Jetted A50 offers a robust dynamic binding capacity of up to 80 g/L, which helps increase product yield compared to other affinity resins.

The resin’s performance is further enhanced by its unique manufacturing process and technical features:

  • Uniform Particle Size: Manufactured using patented Jetting technology, the resin ensures consistent quality and enhanced performance across applications.
  • Alkaline Stability: The resin remains stable in alkaline conditions up to 0.1 M NaOH, ensuring reliable performance during standard cleaning protocols.
  • Faster Time to Market: The Jetting process involves fewer production steps than traditional methods, helping to accelerate your manufacturing timelines.
  • Sustainability Standards: Jetted manufacturing supports eco-friendly operations by reducing the use of harmful solvents and overall water usage.

Purolite - Praesto® Jetted A50 HipH

Praesto Jetted A50 HipH is specifically engineered to solve the stability challenges associated with purifying complex, acid-sensitive molecules like Fc-based IgGs. While these molecules are vital to therapeutic drug development, they often become unstable and aggregate at the typical elution levels of pH 3–3.5 used by standard resins, which significantly decreases product yield. This resin addresses those limitations by enabling elution at much higher levels up to pH 5, thereby enhancing monomer recovery and limiting aggregation. By mitigating protein aggregation early in the screening process, it effectively reduces the burden on downstream polishing steps, simplifying the removal of product impurities.

The technical performance of this resin is supported by several key features:

  • Optimized Capacity: Utilizes a high-capacity 50 µm bead that delivers a dynamic binding capacity of up to 60 g/L.
  • Alkaline Stability: Engineered to remain stable in alkaline conditions up to 0.1 M NaOH, ensuring compatibility with standard cleaning protocols.
  • Superior Manufacturing: Produced using patented Jetting technology, ensuring a uniform particle size for more consistent and enhanced performance.
  • Sustainability and Speed: The Jetting process supports eco-friendly manufacturing by reducing the use of water and harmful solvents while accelerating time to market through a streamlined production workflow.

Praesto™ Jetted SP & Praesto Jetted Q

Praesto Jetted SP and Praesto Jetted Q are high-performance ion exchange (IEX) resins. They are designed to optimize selectivity and capacity for the most demanding separation requirements. As a staple in downstream purification, these resins efficiently remove a broad range of target impurities, including DNA, host cell proteins (HCPs), viruses, and leached Protein A. Both the SP and Q variants are engineered with identical chemical properties across different particle sizes. Hence, they offer tunable performance that allows process engineers to balance capacity, resolution, and flow rate according to specific molecule needs. This robust design supports seamless process scalability while providing the chemical and physical stability necessary for a long resin lifetime and reduced long-term operational costs.

To ensure consistent quality and operational flexibility, these resins include the following features:

  • Versatile Sizing: Available in a wide variety of particle sizes, including 35 µm, 45 µm, 65 µm, and 90 µm.
  • High Performance: Delivers dynamic binding capacities ranging from 50 g/L to 90 g/L.
  • Exceptional Flow:Supports strong flow velocities between 120 cm/h and 550 cm/h.
  • Patented Manufacturing: Produced using Jetting technology to ensure uniform particle size and enhanced performance.
  • Sustainable & Efficient: The manufacturing process reduces the use of harmful solvents and water, while fewer production steps accelerate the overall time to market.

Purolite - Praesto® SP45 / SP65 /SP90

Products for CEX Chromatography

Products for Protein A Chromatography

Agarose Highly Cross-Linked Strong Cation Exchanger, 45 μm particle size Principal Applications: 
  • Protein purification (20-500 kDa)
  • MAb Purification
Advantages:
  • Excellent dynamic binding capacities for high productivity and scalability
  • Outstanding pressure/flow performance
  • High resolution/selectivity
  • Excellent chemical and physical stability
  • Secure, validated supply and regulatory support
  • Enabling the use of a classic antibody purification platform
  • Retaining the unique specificity of Protein A for high recovery and excellent productivity
  • Minimizing impact on facility fit with comparable elution volumes and buffer usage
  • Lowering levels of post-Protein A contaminants, reducing polishing challenges and driving process robustness

Purolite - Praesto® SP35

Products for CEX Chromatography

Products for Protein A Chromatography

Agarose Highly Cross-Linked Strong Cation Exchanger, Uniform Particle Size, 35 μm Principal Applications:
  • Protein purification (20-500 kDa)
  • MAb Purification
Advantages:
  • Manufactured using the patented Jetting technique
  • Uniform particle size
  • Enhanced productivity
  • High dynamic binding capacity
  • Enhanced pressure/flow performance
  • Manufactured with reduced environmental impact
  • Secure, validated supply and regulatory support

Purolite - Praesto® Jetted Q35

Products for AEX Chromatography

Products for Protein A Chromatography

Agarose Highly Cross-Linked Strong Anion Exchanger, Uniform Particle Size, 35 µm Principal Applications:
  • Protein purification (20-500 kDa)
  • MAb Purification
Advantages:
  • Manufactured using the patented Jetting technique
  • Uniform particle size
  • High capacity, enhanced productivity
  • Enhanced pressure/flow performance
  • Manufactured with reduced environmental impact
  • Secure, validated supply and regulatory support

Purolite - Praesto® Q45 / Q65 / Q90

Products for AEX Chromatography

Products for Protein A Chromatography

Agarose Highly Cross-Linked Strong Anion Exchanger, Uniform Particle Size, 35 µm

 

Principal Applications:

  • Protein purification (20-500 kDa)
  • MAb Purification

Advantages:

  • Manufactured using the patented Jetting technique
  • Uniform particle size
  • High capacity, enhanced productivity
  • Enhanced pressure/flow performance
  • Manufactured with reduced environmental impact
  • Secure, validated supply and regulatory support